Tabulation of ABRF-89SEQ results by individual facilities The following table lists the results from the 62 facilities that responded to the 1989 sequence study prior to release of the sequence. The table illustrates the range in sequence assignments from different facilities. These data also suggest that the most common problems in sequence assignment were getting out of phase in later cycles as lag increased and overassignment of residues beyond where the sequence terminated. Tentative resiudes are represented "in lower case, and unassigned residues are represented by a hyphen. If two assignemnts were made for a single " "cycle, both are listed; if more than two assignments were made, the cycle is reported here as unassigned. The first " "column, ""Seq"", is the correct sequence. The C-terminal Cys was unmodified and was not expected to be identified."
Seq 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62
1. D D c D D D D D –4 D D D D D D D D D D D D D D D D D D –8 D D c D D D D D D D D D D D D D d D D dl D D D D D – D N D D D T D D D
2. F F F F F F F F F F F F F F F F F F F F F F F F F F F – F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F F
3. W W W W W W W W w W W W W W W W W W W W W W W W W W W – W W W W W W W –9 W W W W W W W W W rp W W W W W W W W W – W W W – W kw W
4. V V V V V V V V V V V V V V V V V V V V V V V V V V V – V V V V V V V V V V V V V V V V F V V V V V V V V V V V V V V V V V V
5. G G G G G G G G G G G G G G G G G G G G G G G G G G G – G G G G G G G G G G G G G G G G G G G G G G G G G E G G G G G G G G G
6. A A A A A A A A A A A A A A A5 A A A A A A A A A A A A – A A A A A A A A A A A A A A A A A A A A A A A a A A A A A A A A A A A
7. H H A H H H H H H – H H H H A H H H H H H H H H H H H – H H H H H H H H H H H H H H H H H H H H H H H h H H H – H H H H H F H
8. A A A A A A A A A A A A A A H A A A A A A A A A A A A – A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A A
9. R R R R R R R R R – R R R R R R R R R R R R R R R R R – R R R R R R R R R R R R R R R R R R R R R R R R R R R – R R R R R R R
10. Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y – Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y Y
11. V V V V V V V V V V V V V V V V V V V V V V V V V V V – V V V V V V V V V V V V V V V V V V V V V V V V V V V V V V V V V V V
12. K K K K K K K K K V K K K K K K K K K K K K K K K – K – K K K K K K K K K K K K K K k K K N K K K K K K K k K K K K K – K K W
13. M M M M M M M M M M M M M M M M M M M M M M M M M M M – M M M M M M W M M M M M M M M M M M M M M M M M M M M M M M M M M M M
14. Q Q Q Q Q Q Q Q Q Q Q Q Q Q Qe Q rp7 Q Q Q Q Q Q Q Q Q Q – Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q
15. E E E E E E E E E E E E e2 E E E6 – E E E E E E E Ed E E – E E – E E E – E E E E E E E E E E E E E E E E E E E E e E E E E E Q E
16. Q Q qp Q Q Q Q Q Q Q Q Q Q3 Q Q – Q Q Q Q Q Q Q Q Q Q Q – Q Q Q Q Q Q – Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q – Q Q Q Q Q Q Q – Q
17. Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q – Q Q Q Q Q Q Q Q Q Q Q – Q Q Q Q Q Q – Q Q Q Q Q Q Q Q Q Q P Q Q – Q Q Q Q D Q Q Q Q Q R Q Q Q
18. V V V V V V V V V V V V V V V – D V V V V V V V V V V – V V V V V V – V V V V V V V V V V rs V V V V V V V A V V V V V V V V V
19. Q Q – Q Q Q Q Q v Q q Q Q Q Q – V Q Q Q Q Q Q Q Q – Q – Q Q Q Q Q Q – Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q Q – Q Q Q Q Q – Q V Q
20. P P P P P P P P p P P P P P P – P P P P P P P P P P P – P P P P P P – P P P P P P P –10 P P P P P P P P P P – P P P P P P P P P
21. S S S' S S S S S S S S ip S S s – P S S S S C hs S D S S – S S S S S S – S S S S S S S p S S – S S P S S S S – S – S S S R s P S
22. R R – R R R R R R N R R R R R – s R R R R R R R s R R – r R R R R R – R S R R R R R R Y R – R R R R R R R – R – R R R Y R P R
23. R R – R R R R R R R R R R R E – R R R R R R – R – R R – r R R R r R – R R r – R R R r Y R – R R R R R r R – R – R R r s R S R
24. S S – S S S S S S H S – S S' S – s S S S R C hs S – D S – s S S S s S – S S s r S S S s S S – S S S S S S G – S – S S R – s G –
25. V V – V V V V V V V V1 V V V V – S V V V V V V V – S V – V V V V V V – V V V V V V V V V V – V V V V V V V – V – V V V V V V V
26. P P – P P P P P P P –– P P P P – V P P P P P p P – – P – P sq P P P P – P P P P P P P P P P – P P P P P P P – P – P P P P P s P
27. N N – N N N N N N S –– N N N N – p N N N R N – N – – N – I N N N N N – N N N N N N N N N N – N N N N N N N – N – N N N k N – N
28. T T – T T T T N T R –– n t T T – N T T T t N – T – N N – – T nt t t T – T T T T n T T N T t – T nt T T T T T – T – T T T l T N nt
29. L L – L L L L L L L – I L L L – T L L L L L – L – V L – L L lt L L L – L L L L L L L D L L – L L L L L L L – L – L L L k L P kl
30. D L – D D D D – D r – – D D D – I D D D i D – D – – D – – D dl D L D – D D D D D D D s' D d – D D – D D D w – D – D D – r H L hd
31. H h – H A H H – H r – – H h H – D H v H G H – H – – H – – h H c D E – – W i H H H H V A h – H H D H H d T – H – H H D – D L ks
32. V V – V V V V – V l – – v v V – – V v V V V – V – – V – V V hv v – V – V V h V V V V – V V – V V V V V V V – V – V V – – V – vd
33. P V – P P P P – P v – – p P P – V P P P P P – – – – T – – P hp p P P – P S V P P P P i P p – P p P P P P P – P – P P D – P – –
34. I p – I I I I – I – – – ei i I – – I i I I I – – – – I – – I P i id I – I R I I I I I i I I – p i – I I – – – I – I I V – I – I
35. I I – I I I I – I – – – i I I – – I p I I I – – – – i – – I I i I I – R I P R I I I i I i – I i I I – I D – I – I I i – I – I
36. S I – S S S S – s – – – t S' S – – S – s S I – – – – – – – is S i I S – i W – – S S S M S – – – i – i s – – – S – I I – – r – –
37. R S – R R h s – r – – – – – R – i R i R H R – – – – – – – – I r – R – r R i S R R R r – – – – r – R r – – – R – – – r – c – –
38. R – – r H R r – h – – – – – r – I e – r L R – – – – r – – – R r – R – d S p – R R r – – – – – – R r – – – – R – – – – – h – –
39. V R – v V w v – V – – – – – V – p v – s V – – – – – – – – – R – – V – – R r R V – v – V – – – – D v – – – – V – – – w – p – –
40. C v – – V f W – – – – – – – – – I – – – V – – – – – v – – – – – – – – – H r – v – – – – – – – – – r – – – – – – – – R – Y – –
41. – – – –– – – vr – i – – – – – – – – – – – – – – – – – – – – – – – – – – – – v – – – – – – – – – – – s – – – – – – – – – – S – –
42. – – – –– – – an – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – – H – – – – – – – – – – p – – – – – – – – – – h – –
1-Instrument failure after cycle 25 due to operator error.
2-Software bug aborted run, manual injection of cycle 15 on 120A performed.
3-Sequencer restarted.
4-No injection at this cycle.
5-Manual injections done at cycles 6-10 and 19-23.
6-Building-wide power failure occured after cycle 15.
7-Power failure at cycles 14 and 15.
8-Major machine malfunction precludes sequencing any samples.
9-Unknown amino acid; between DPTU and DPU.
10-Malfunction at cycle 20.
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Created: 10th August 1995
Last modified: 21st October 1995