Tabulation of ABRF-90SEQ results by individual facilities.
The following table lists the results from the 56 facilities that responded to the 1990 sequence study prior to release of the sequence. The table illustrates the range in sequence assignments from different facilities. Tentative residues are represented in lower case, and unassigned residues are represented by a hyphen. If two assignments were made for a single cycle, both are listed; if more than two assignments were made, the cycle is reported here as unassigned. The residue listed to the right of the cycle number is the correct sequence. The C-terminal Cys was cross-linked to a carrier protein (transferrin) and was not expected to be identified. A detailed evaluation of this data is published in "Techniques in Protein Chemisty II (J. Villafranca, ed.), Academic Press, in press."
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56
Sequencer 5 0 7 P 0 7 0 5 7 5 7 0 0 5 5 7 7 5 0 0 5 M 7 0 0 0 0 7 P 7 P 0 0 0 7 7 0 7 5 – 7 5 0 0 0 0 7 7 0 0 3 7 0 7 0 7
Age (yrs.) 5 4 1 2 6 2 6 8 2 8 3 7 5 7 5 2 4 5 6 7 7 1 2 6 5 6 6 2 0 4 1 4 7 4 2 1 5 2 8 – 3 – 7 5 5 5 1 3 5 4 0 1 6 3 8 3
Mode L G L G G G G G L L L G G L L L L G G G L S L G G G G L G L G G G G G L G L G - L L G G G G L L L G L L G L G L
Glass Filter B B B N N B A B N B B B A B B B B B N A B N B B B B B B N B N B B Y B B N B N – B B N B B N B B N B A B B A A B
Polybrene N Y Y – – Y Y Y – Y Y Y Y Y Y Y Y N – Y Y – Y Y Y Y Y Y – Y – Y N Y Y Y – Y – – Y Y – N Y – Y Y – N Y Y Y Y Y Y
Cycle #
1. Y Y s –4 Y Y Y Q Y Y – – Y Y I Y Q K Y Y Y Y – R – F – – Y Y Y Y Y Y yv Y yv yh Y Y Y Y Y Y p Y Y Y Y – Y Y Y R Y – Y
2. L L L – L L L L G L L L L L L L s L L L L L L L L L L L L L l L L L L L L L L L L L L L L – L L L L L L L L L L L
3. D D R – D D D D Q D D D D D R D I cs D D D D D Y D G G c D D D D di D D A lv D D D D D D D D s D D D D D D D d c – D
4. N N N – N N N N N N N N N N N N N N N N N W N A N D N N N N N N N N nk N N N N N N N N N N N N N N N N S N n N – wr
5. D D D – D D D D S D D – D dt D D D D D D D T D A D N p D T D D D dt D dt – – D D D T d – D d T T D T D N T D d D D A
6. W H D – W – c – – –5 v – w wv V v T V W w h M V I V W k D V W F W xv w wv V – V W – W – W W V W – –* W W – I – – V sv T
7. T T A – T t T T – – – – T T T T cw T T T T T e I T T T T T T T T T T T T T – T T T T T T t T T T T T T T – – T – T
8. S R S – – – S S – – – – s S ys S cw R S S s S S A S S s s S S s S S S S S – – S – sl – – S – – S S – T s S – – S S S
9. A A – – A – A A A – – A A A A A A A A A A A A A A A A A A A a A A A A A A P A – A A A A A A A A A A Q A – – A al A
10. L S – – L – L L L – – – L L L L L L L L L L L – L L H L L L l L L L L L L A L – L L L L l – L L L L – L – – L – L
11. R A – – R – R – – – – – – – – R R R r r R L R I G N L L r R – R R – – M r L R – – P R r V – – wc – – L – – – L – H
12. V L – – V – V V V – – V V V V V V V V V V V V V V V V v V V f V V V V V V – V – V V –* V v V V v* V V – V – – V V V
13. M V – – M – M – M – – M M M M M M M M M M M M M M M m M M M m M M M M M M – M – M M M M M M – M M M – M – – M M M
14. P P – – P – P P P – – P P P rp P P P P P P P T P P D p P P P p P P P P P P – P – P P P P P p P P P – – P – – P P H
15. S R – – – – – – – – – D k S P S S hd S s – k S A S C – s A S s s S S S S P – S – – pk – s – S S S S – – s – – S S P
16. I H – – I – – – S – – K I I Q I I I I I – i I – I I – I I I f I I I I I – – I – I I I I I I I i* I – – – – – I – I
17. L I – – L – – – – – – – – L lw L H L L L – – T dl L L – l T L l L L – L L L – L – L L – L – – L L L – – L – – L – L
18. K – – – K – – – – – – – l – K K I K k H – – G – W R – K L K s – K – K – k – K – K K – K – – k K K – – K – – K – H
19. V K – – V – – – – – – – V V T V V V V V – – V – V Q – V V V v V V v V P – – V – V V – V – V – –* V – – V – – V – L
20. Q R – – Q – – – – – – Q k Q Q Q – P Q Q – – Q – Q G R Q Q Q q – Q q Q R – – Q – Q – – Q m – q Q Q – – D – – Q – Q
21. G – – – G – – – – – – – – G H G – V g G – – – – G Q q g g G – – G – G – V – G – G g – g – – f G G – – eg – – G – G
22. R – – – R – – – G – – – – – D – – Q – – – – T – – R – r d R – – R – – – E – r – – – – – – – – – I – – – – – G – R
23. E – – – – – – – R – – – – E – E – – e – – – E – E – – e e E – – E – E – – – e – – – – e – – – E E – – – – – N – c
24. V – – – – – – – – – – – – V – V – – v – – – e – V – – V v V – – V – V – K – V – – – – – – – – V – – – – – – V – Q
25. S – – – – – – – – – – – – – – – – – r – – – S – – – – n d S – – S – – – – – S – – – – – – – – – – – – V – – V – D
26. H – – – – – – – – – – – – – G – – – – – – – – – – – – K s H – – h – – – – – h – – – – – – – – – – – – – – – A – L
27. F – – – – – – – – – – – – F – F – – f – – – F – – – – – v F – – F – F – – – F – – – – – – – – F – – – – – – F – H
28. K – – – – – – – – – – – – – – – – – s – – – – – – – – – p t – – K – K – D – K – – – – – – – – – – – – – – – – – F
29. C – – – – – – – – – – – – – – – – – – – – – S – – – – – g R – – – – – – – – d – – – – – – – – – – – – – – – – – S
30. – – – – – – – – – – – – – – – – – – – – – – W – – – – – t h – – – – – – – – g – – – – – – – – – – – – – – – – – c 1-Sequencer: 0 = ABI 470AG 3 = ABI 473A 5 = ABI 475A 7 = ABI 477A M = MILL 6600 P = POR 2090E 2-Mode G = gas phase L = pulsed liquid phase S = solid phase 3-Glass Filter A = above PVDF B = below PVDF N = none Y = yes, location not indicated 4-Injection line from ABB 477 to ABI 120 clogged during run. No data were collected. 5-Machine failure, no data collected. 6-Injection failure.
Return to the The ABRF Home Page
Created: 21st October 1995
Last modified: 21st October 1995