The 1994 study will evaluate specific cleavage methodologies used after peptide synthesis. These specific cleavage methodologies are detailed in a letter and forms which were sent to all ABRF members in February. Members are asked to synthesize a designated peptide sequence employing the chemistry most often used in their facility. Specific side chain protecting groups and a choice of two cleavage and deprotection protocols are given. The committee hopes to determine which cleavage and deprotection protocol yields greater amounts of the desired product. This study is in contrast to last year when ABRF facilities submitted peptides to the committee which had been cleaved and deprotected under a variety of conditions. Members are asked to supply 5 mg of crude dry product in a 1.5 ml Eppendorf tube which will be analyzed by laser desorption and electrospray mass spectrometries, by amino acid analysis, reversed-phase high performance liquid chromatography and finally (selected samples) sequence analysis.
The 1994 study was designed based on the results of the 1993 study when ABRF members were given protected peptide-resin to be cleaved and deprotected by techniques used in their facility and returned to the committee in a crude desalted form for analysis. From the 1993 study it appeared that certain methodologies yielded the most favorable results, and therefore these methodologies are to be compared and evaluated in the 1994 study. Analysis of this year's study should be completed in time for presentation at the annual ABRF meeting on July 9th, 1994.
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