Peptide Synthesis Research Committee
In its 1995 study, the peptide synthesis research committee of the ABRF
will depart from the direction of previous years, in which tests were made
of synthesis, cleavage, and workup procedures routinely used in our
facilities. The present study will test the effectiveness of protocols for
formation of intrachain disulfide bonds. Member laboratories will be asked
to synthesize a short peptide and then to oxidize the sulfhydryl groups to
form the disulfide bond. There will be a choice of four protocols, two
on-resin and two post-cleavage reactions. Each participating laboratory can
perform only one, or all four methods, if desired. The laboratories will be
asked to return unpurified peptide for testing by HPLC, amino acid
analysis, mass spectrometry, and spectrophotometric analysis by Ellman's
reagent. All samples will be coded for confidentiality. Although these
procedures are rarely a part of routine services, they are ever more
frequently needed for research projects. The design of this year's study
will not only test our abilities but will provide an opportunity for us to
efficiently incorporate these technologies into our laboratories.
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Created: 27th July 1995
Last modified: 27th July 1995