This international, three-day symposium will focus on current and emerging methods related to protein, peptide, carbohydrate, and nucleic acid synthesis and analysis. Concurrent sessions during most of the meeting will ensure that scientists interested in a single type of biomolecular technology should find topics of interest throughout the meeting. The meeting will be held at the Holiday Inn Golden Gateway in San Francisco, CA from March 30 to April 2, 1996.
Saturday, March 30
Opening Reception (7:00 p.m.-9:00 p.m.) Emerald Room
Sunday, March 31
Complimentary Continental Breakfast (8:00 a.m.) Convention Foyer, Lower Level
Plenary Session: Impact of the human genome project on biotechnology (9:00 a.m.) Leroy E. Hood, Genomics and large-scale analyses of biological and medical challenges, Emerald Room
Exhibits Open (10:00 a.m.-6:00 p.m.) Gold Rush and Crystal Rooms
Posters Open (10:00 a.m.-6:00 p.m.) Gold Rush, Crystal, and Redwood Rooms
Coffee Break (10:15 a.m.) Convention Foyer
Concurrent Sessions (10:50 a.m.)
* Workshop 1: Internal Edman sequencing of SDS PAGE-separated proteins, chaired by Kenneth R. Williams, Emerald Room
- Kenneth R. Williams, In-gel enzymatic digestion of proteins for Edman degradation
- Sheenah M. Mische, Enzymatic digestion of PVDF-bound proteins
- Scott D. Patterson, On-membrane digestions for subsequent mass spectrometric and amino acid sequence analysis using Immobilon-CD
* Workshop 2: Automating genomic applications, chaired by Elizabeth Nanthakumar, Oregon/Nevada Rooms
- Elizabeth Nanthakumar, Automated fluorescent genotyping
- Lance Fors, Mutation detection and localization and genetic identification using cleavage fragment length polymorphism
* Tutorial 1: Improving the art and practice of amino acid analysis background, chaired by John W. Crabb, California Room
Lunch Break (12:20 p.m.)
Exhibits Open (10:00 a.m.-6:00 p.m.), Gold Rush and Crystal Rooms
Posters Open (10:00 a.m.-6:00 p.m.), Gold Rush, Crystal, and Redwood Rooms
Concurrent Sessions (1:30 p.m.)
* Workshop 3: Subpicomole Edman sequencing, chaired by William J. Henzel, Emerald Room
- William J. Henzel, Subpicomole sequencing using capillary PTH analysis
- Paul Tempst, Innovative versus practical sides of subpicomole sequencing
- Ruedi Aebersold, Subpicomole protein sequencing
* Workshop 4: Capillary electrophoresis of proteins and peptides, chaired by Alan Smith, Oregon/Nevada Rooms
- James Landers, Peptide and protein analysis by capillary electrophoresis: theory and practice
- Werner J. Kuhr, On-line peptide mapping with microreactors coupled to capillary electrophoresis and mass spectrometry
- John Kelly, Practical considerations for the analysis of peptides and proteins by CZE-MS
* Tutorial 2: Interpreting automated DNA sequences and sequencer problems, chaired by Duane A. Bartley, California Room
Refreshment Break (3:00 p.m.) Gold Rush and Crystal Rooms
Concurrent Sessions (3:15 p.m.)
* Workshop 5: Strategies for expressing and purifying recombinant proteins, chaired by David W. Speicher, Emerald Room
- Christopher S. Jones, Use of affinity tag technology for purification of recombinant proteins
- David W. Speicher, Optimizing immunoaffinity purification of soluble and membrane-associated recombinant proteins
* Roundtable Discussion 1: Improving the status of core laboratories and personnel, chaired by Karen S. De Jongh, Washington Room
* Tutorial 3: Analysis of carbohydrate content of glycoproteins, chaired by Roberta K. Merkle, California Room
Poster Session 1 (4:30 p.m.) Redwood, Gold Rush, and Crystal Rooms
Monday, April 1
Complimentary Continental Breakfast (7:30 a.m.) Convention Foyer, Lower Level
Symposium: Quantitative analysis of macromolecular shape and interactions: (re)emerging thermodynamic and kinetic approaches, chaired by Preston Hensley, (8:30 a.m.) Emerald Room
- Preston Hensley, The use of the analytical ultracentrifuge to characterize the interactions of macromolecules in solution
- Michael L. Doyle, Applications of titration microcalorimetry with biopharmaceuticals
- Maurice M. Morelock, Determination of receptor-ligand kinetic and equilibrium binding constants using surface plasmon resonance
Exhibits Open (10:00 a.m.-6:00 p.m.) Gold Rush and Crystal Rooms
Posters Open (10:00 a.m.-6:00 p.m.) Gold Rush, Crystal, and Redwood Rooms
Coffee Break (10:30 a.m.) Convention Foyer
Concurrent Sessions (10:50 a.m.)
* Workshop 6: Current peptide synthesis methods and the potentials of combinatorial chemistries, chaired by Gregory Grant, Emerald Room
- George Barany, Current strategies for disulfide formation in synthetic peptides
- David Shaw King, Strategies for the synthesis of large peptides
- Michael Lebl, Peptide and non-peptide combinatorial libraries
* Workshop 7: Characterization of glycosylated proteins on gels and blots, chaired by Reid Townsend, Oregon/Nevada Rooms
- R. Reid Townsend, Analysis of proteins on PVDF membranes for carbohydrate content
- Robert B. Trimble, Characterization of glycans on glycoproteins resolved on SDS gels and blotted onto membranes
* Tutorial 4: Sequencing difficult DNA templates and PCR products, chaired by Margaret A. Robertson, California Room
Lunch Break (12:20 p.m.)
Exhibits Open (10:00 a.m.-6:00 p.m.) Gold Rush and Crystal Rooms
Posters Open (10:00 a.m.-6:00 p.m.) Gold Rush, Crystal, and Redwood Rooms
Concurrent Sessions (1:30 p.m.)
* Workshop 8: Identification and significance of post-translational modifications, chaired by Kenneth A. Walsh, Oregon/Nevada Rooms
- Kenneth A. Walsh, Inspecting cages in the post-translational zoo
- Julian D. Watts, Determination of sites of reversible protein phosphorylation
- Robert S. Rush, Microheterogenity of erythropoietin carbohydrate structure
* Workshop 9: DNA sequencing methods--current status and future prospects, chaired by Alan Smith, Emerald Room
- J. William Efcavitch, Capillary electrophoresis and DNA analysis
- Norman J. Dovichi, Multiple capillary DNA sequencing
- Lloyd M. Smith, High-speed DNA sequencing: progress and prospects
* Tutorial 5: Efficient electroblotting and on-membrane proteolysis at high sensitivity for sequence analysis, chaired by David W. Speicher, Washington/California Room
Concurrent Sessions (3:15 p.m.)
* Workshop 10: C-Terminal sequencing methods, chaired by John E. Shively, Emerald Room
- John E. Shively, Overview of methods for C-terminal sequence analysis of proteins
- Victoria L. Boyd, Chemical side-reactions during C-terminal sequencing
- Jerome M. Bailey, Automated thiohydantoin chemistry for C-terminal sequence analysis
* Tutorial 6: Interpretation of MS and MS-MS data, chaired by Roland S. Annan, Washington/California Room
Poster Session II (4:30 p.m.) Redwood, Gold Rush and Crystal Rooms
Tuesday, April 2
Complimentary Continental Breakfast (7:30 a.m.) Convention Foyer, Lower Level
Symposium: Present status and future prospects of MS for analysis of biomolecules, chaired by Steven A. Carr, (8:30 a.m.) Emerald Room
- Matthias Mann, Advanced uses of biological mass spectrometry in a core facility
- Brian T. Chait, Matrix-assisted laser desorption ion trap mass spectrometry as a tool for protein identification and characterization
- Steven A. Carr, The essential roles of mass spectrometry in characterizing protein biopharmaceuticals
Exhibits Open (10:00 a.m.-2:00 p.m.) Gold Rush and Crystal Rooms
Coffee Break (10:30 a.m.) Convention Foyer
Concurrent Sessions (10:50 a.m.)
* Workshop 11: Identification of novel proteins from gels and blots using MS, chaired by Darryl Pappin, Emerald Room
- Darryl Pappin, Peptide-mass fingerprinting by linear TOF: the fast, cheap way to identify electroblotted proteins
- John R. Yates, Automated protein mapping with tandem mass spectrometry and Sequest
- Matthias Mann, Identification of proteins from polyacylamide gels by peptide sequence tags
* Workshop 12: GLP compliance and validation, chaired by John E. McEntire, Oregon/Nevada Rooms
- John E. McEntire, Regulatory compliance in the bioanalytical laboratory
- John E. Dougherty, Assay control systems in the bioanalytical laboratory
* Tutorial 7: In-gel protein digestion at high sensitivity, chaired by Kathryn L. Stone, California Room
Lunch Break (12:20 p.m.)
Concurrent ABRF Research Committee Reports (1:30 p.m.)
Emerald Room Oregon/Nevada Rooms Time
Protein Sequencing DNA Sequencing 1:30 p.m
Internal Protein Sequencing Nucleic Acids 1:55 p.m
Amino Acid Analysis GLP/cGMP 2:20 p.m
Peptide Synthesis Survey 2:45 p.m
Carbohydrate Analysis 3:10 p.m.
Refreshment Break (3:35 p.m.) Oregon/Nevada Room
Concurrent Sessions (3:45 p.m.)
* Workshop 13: Methods for capillary HPLC isolation of peptides, chaired by Kristine M. Swiderek, Emerald Room
- Kristine M. Swiderek, Fast screening of protein samples by automated microspray LC-MS/MS analysis: a practical application for core facilities?
- Axel Ducret, Capillary LC-ESI-MS/MS for the identification of proteins with known sequence and de novo protein sequence analysis
- Daniel B. Kassel, Multi-dimensional HPLC-MS methods for optimizing receptor:ligand and enzyme:substrate binding
* Workshop 14: Efficient handling of samples and data in high-volume nucleic acids laboratories, chaired by Elizabeth Nanthakumar, California Room
- John Rush, Multiplex DNA synthesis--high-throughput, low-cost oligonucleotides
- Trevor Hawkins, Integration of laboratory automation for the human genome project: the Sequatron
- Elizabeth Nanthakumar, Database development for the core laboratory
* Roundtable Discussion 2: Merits of alternative MS methods--which type of instrument should I buy?, chaired by John T. Stults, Oregon/Nevada Rooms
ABRF Business Meeting (5:15 p.m.) Emerald Room.
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