Created: 19th June 1998, last updated: 19th June 1998, © 1998 ABRF
Research Group/Committee Reports
Peptide Synthesis Research Group
The Peptide Synthesis Research Group (PSRG) is currently completing the analysis of the results from its 1997/1998 study. The purpose of this study was to assess the ability of member facilities to identify and solve problems in peptide synthesis and to identify the methods used. The first test peptide sent to facilities (peptide 1) was synthesized in the reverse order with two amino acid substitutions that would give a mass identical to the "requested" peptide. The second test peptide (peptide 2) was made to represent an oxidation event. This peptide contained 70% non-oxidized methionine and 30% oxidized methionine. Both test peptides, but especially peptide 1, appeared very susceptible to deamidation of the carboxy-terminal amide. The results of this study are still being compiled and analyzed and will be available to participating laboratories in August, 1998. We would like to thank all of the laboratories who participated in this study.
We are currently testing the design of our 1998/1999 study on peptide modifications. Specifically, the PSRG will be assessing the ability of member laboratories to produce a biotinylated peptide. This study will begin in September in order to report results at the ABRF '99 meeting. We look forward to your participation in this study.