Created: 1st December 1999, last updated: 21st February 2000, © 1999 ABRF
Matsui, Takuro, 194
Admon, Arie, 26
Andersen, T. T., 129
Angeletti, Ruth Hogue, 2, 155
Anumula, Kalyan R., 144
Ballard, Linda Wood, 177
Bao, Y., 129
Begg, Gillian E., 17
Bonner, M. Roxane, 177
Burgess Antony W., 64
Cohen, S. A., 129
Cole, James L., 163
Crabb, J. W., 129
deBlaquiere, Julie, 64
Denslow, N. D., 129
Fernandez, Joseph, 26
Folta-Stogniew, Ewa, 51
Fox, Jay W., 187
Gove, Deborah S., 11
Gunthorpe, Martha, 187
Hager, Karl M., 187
Hansen, Jeffrey C., 163
Henzel, William, 26
Hulmes, J. D., 129
Hunziker, P., 129
Keating, Kathleen M., 72
Lane, William, 26
Lilley, Kathryn S., 187
Mahrenholz, A. M., 129
Mann, K., 129
Mardis, Elaine R., 137
Michnick, Stephen W., 32
Mozdzanowski, Jacek, 144
Novick, Peter, 149
Naeve, Clayton, 1, 153
Pelletier, Joelle N., 32
Pitt, Aldo M., 21
Remy, Ingrid, 32
Rohde, Michael, 26
Sakai, Tomoko, 194
Schegg, K. M., 129
Sheer, G. Donald, 21
Speicher, David W., 17
Steinke, Laurey, 26
Stone, Kathryn, 26
Takayama, Mitsuo, 194
Tang, Sheng, 144
TerBush, Daniel R., 149
Tsugita, Akira, 194
Watson, Jack Throck, 75
West, K. A., 129
Williams, Kenneth R., 51
Wu, Jiang, 75
Yang, Ying, 75
Yeung, Anthony, 187
ABI 473A Sequencer, 144
acetylisothiocyanate, 144
acid hydrolysis, 194
affinity purification, plasma membrane, 64
amino acid thiohydantoin, 144
amino acids
analysis of, 129
identification of D-, 72
analytical centrifugation, 163
anti-peptide antibodies, 2
antibodies, sequence specificity of, 2
assembly mechanisms, determination by analytical centrifugation, 163
Association of Biomolecular Resource Facilities (ABRF)
ABRF-97SEQ sequencing results, 26
awards of, 43, 83
Molecular Interactions Research Group of, 86
multicenter study for protein identification, 129
Nucleic Acids Research Group (NARG) market survey for, 187
biotin, membrane purification with, 64
capillary electrophoresis platforms, 137
carboxyl-terminal sequencing, 144, 194
carrier proteins, 2
Caruthers, Marvin, ABRF award to, 83
cell-surface membranes, affinity purification of, 64
centrifugation, analytical, 163
chiral modifications in synthetic peptides, 72
chromatography, size exclusion with, 51
cleavage, specific, 75
conformation polymorphism, analysis of, 177
conformational changes, characterization by analytical centrifugation, 163
core facilities
ABRF evaluation of, 26
market survey update for, 187
quantitative real-time polymerase chain reaction for, 11
sequencing methods for, 177
cost
of denaturing high-performance liquid chromatography, 177
of investment in intellectual resources, 154
of multicapillary DNA sequencing instruments, 137
of National Cancer Institute resource laboratories
of quantitative real-time polymerase chain reaction method, 11
of single-strand conformation polymorphism, 177
cyanylation, 75
cysteine, identification of, 17, 26
denaturing high-performance liquid chromatography (DHPLC), 177
dihydrofolate reductase, 32
disassembly mechanisms of biomolecular complexes, 163
disulfide adduct, detection of, 17
disulfide bond pairing, 75
DNA
capillary electrophoresis platforms for sequencing, 137
market survey update of facilities synthesizing, 187
quantitative real-time polymerase chain reaction for sequencing, 11
trends in synthesis at core facilities
DNA Sequencing Research Group (DSRG), 86
enzyme reconstruction, 32
enzymes, chiral specificity of, 72
epidermal growth factor (EGF) receptor, 64
epitopes, sequence, 2
equilibrium constants, 163
Exocyst, 149
funnel tube gels, 149
fusion proteins, purification of, 17
gene sequencing, quantitative real-time polymerase chain reaction for, 11
genomic variation, detection of, 177
hetero-associating systems, characterization by analytical centrifugation, 163
high-performance liquid chromatography (HPLC)
denaturing, 177
ion-pair reversed-phase, 177
size exclusion with, 51
sample preparation for, 21
high-throughput DNA sequencing, multicapillary instruments for, 137
high-throughput instrumentation for synthesis, 187
high-throughput screening, 21
hydrodynamic parameters, 163
ion-pair reversed-phase high-performance liquid chromatography, 177
laser light scattering, 51
leucine zipper, 32
macromolecular conformational changes, detection by analytical centrifugation, 163
mass spectrometry
disulfide adduct detection with, 17
protein folding identification with, 75
structural characterization with, 21
use in ABRF-97SEQ evaluation, 26
matrix-assisted laser desorption and ionization/time of flight (MALDI-TOF) mass spectrometry
carboxyl-terminal sequencing analysis with, 194
disulfide adduct detection with, 17
MegaBACE 1000 DNA Sequencing System, 137
microplate format, 96-well, 21
Molecular Interactions Research Group, ABRF, 86
molecular mass determination, 163
molecular weight determination, 51
multicapillary electrophoresis, 137
multiple synthesizers, 187
MultiScreen equipment, 21
mutation detection, 177
native molecular weight, 51
Nucleic Acid Research Group (NARG)
new chair of, 87
market survey update for, 187
nucleic acids, sequence quantification of, 11
oligomerization determination, size exclusion chromatography and laser light scattering for, 51
oligonucleotides, synthesis of, 187
peptide antigens, design of, 2
Peptide Synthesis Research Group (PSRG), 44
Peptide Synthesis Research Group, 156
peptide, proteases for identification of, 72
peptide, structural characterization of, 21
peptides
ABRF-97SEQ evaluation of, 26
acid hydrolysis of, 194
chiral specificity of, 72
synthesis of, 2
Perkin Elmer/Applied Biosystems 7700 Sequence Detector, 11
Perkin-Elmer 3700 DNA Analyzer, 137
polyvinylidene difluoride (PVDF) membrane, protein immobilization on, 129
polymerase chain reaction (PCR)
genome analysis with, 177
quantitative real-time, 11
proteases, for identification of chiral modifications in synthetic peptides, 72
proteins
ABRF-97SEQ evaluation of sequencing of, 26
amino analysis in identification of, 129
association of, 51
carboxyl-terminal sequencing of, 144
carrier, 2
chemistry for, 51, 144
design of, 32
folding of, 75
funnel tube gel concentration of, 149
nucleic acid interactions with, 51
polyvinylidene difluoride membrane immobilization of, 129
recombinant fusion, 2
protein-fragment complementation assay, 32
proteome analysis, 64
purification
antibody, 2
affinity, 64
fusion protein, 17
quantitative real-time polymerase chain reaction, 11
racemization, amino acid, 72
recombinant fusion proteins, 2
recombinant proteins, disulfide adducts of, 17
reducing agents for disulfide adducts, 17
refractive index, molecular weight determination with, 51
RNA, quantitative real-time polymerase chain reaction for, 11
Saccharomyces cerevisiae, 149
sample preparation, high-throughput, 21
sample purity, determined by analytical centrifugation, 163
SEC genes, 149
secretory pathway, 149
sedimentation equilibrium, 163
sedimentation velocity, 163
self-associating systems, characterization by analytical centrifugation, 163
sequencing
ABRF-97SEQ evaluation of, 26
quantitative real-time polymerase chain reaction for, 11
signal transduction, 64
single nucleotide polymorphisms (SNPs), 177
single-strand conformation polymorphism (SCCP), 177
size exclusion chromatography, 51
slab gels, 137
solution interactions, 163
subunit stoichiometries, determination by analytical centrifugation, 163
synthesis protocols, market survey of, 187
synthesizers, multiple for core facilities, 187
TaqMan probe, 11
Taq polymerase, 11
thermodynamic parameters, 163
thiohydantoin amino acid, 144
trapping folding intermediates, 75
tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl), 17
tryptophan, identification of, 26
ubiquitin, 32
ultraviolet light, molecular weight determination with, 51