Calculation Example: Residues per Molecule-Known
| DATE RUN |
3/31/96 |
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| NAME/ID # |
KNOWN (NBS-BSA) |
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| VOL HYDROLYZED |
10 |
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| RATIO APPLIED |
1.00 |
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| Molecular weight |
66328 |
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| AMINO ACID |
known composition |
pmol analyzed |
PMOL PROTIEN |
OVER 15% |
UNDER 15% |
EXP COMP |
INT COMP |
% INT ERR |
| ASP (D) |
54 |
1606.1 |
29.74 |
29.74 |
29.74 |
56.9 |
57 |
5.56 |
| GLU (E) |
79 |
2251.26 |
28.50 |
28.50 |
28.50 |
79.7 |
80 |
1.27 |
| SER (S) |
28 |
760.99 |
27.18 |
27.18 |
27.18 |
26.9 |
27 |
3.57 |
| GLY (G) |
16 |
545.79 |
34.11 |
FALSE |
FALSE |
19.3 |
19 |
18.75 |
| HIS (H) |
17 |
469.49 |
27.62 |
27.62 |
27.62 |
16.6 |
17 |
0.00 |
| ARG (R) |
24 |
637.16 |
26.55 |
26.55 |
26.55 |
22.6 |
23 |
4.17 |
| THR (T) |
34 |
887.22 |
26.09 |
26.09 |
26.09 |
31.4 |
31 |
8.82 |
| ALA (A) |
46 |
1316.07 |
28.61 |
28.61 |
28.61 |
46.6 |
47 |
2.17 |
| PRO (P) |
28 |
832.03 |
29.72 |
29.72 |
29.72 |
29.5 |
30 |
7.14 |
| TYR (Y) |
19 |
621.56 |
32.71 |
FALSE |
FALSE |
22.0 |
22 |
15.79 |
| VAL (V) |
36 |
967.31 |
26.87 |
26.87 |
26.87 |
34.2 |
34 |
5.56 |
| MET (M) |
4 |
112.87 |
28.22 |
28.22 |
28.22 |
4.0 |
4 |
0.00 |
| ILE (I) |
14 |
298.79 |
21.34 |
21.34 |
FALSE |
10.6 |
11 |
21.43 |
| LEU (L) |
61 |
1844.82 |
30.24 |
30.24 |
30.24 |
65.3 |
65 |
6.56 |
| PHE (F) |
27 |
799.26 |
29.60 |
29.60 |
29.60 |
28.3 |
28 |
3.70 |
| LYS (K) |
59 |
1102.34 |
18.68 |
18.68 |
FALSE |
39.0 |
39 |
33.90 |
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| TOTAL #AA |
546 |
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NEW ESTIMATE |
PMOL PROTEIN |
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| ESTIMATED AMOUNT |
ANALYZED |
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WITHIN 15% |
28.25 |
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AVERAGE % |
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| PMOL PROTEIN |
27.86 |
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ERROR |
8.65 |
| 15% OF PROTEIN |
4.18 |
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TOTAL PMOL |
AMINO ACID |
15053.06 |
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| -15% OF PROTEIN |
23.68 |
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TOTAL |
PMOL HYDROL |
28.25 |
Total ugrams |
1.87 |
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| +15% OF PROTEIN |
32.04 |
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CONC pmol/µl |
2.825 |
CONC µg/µl |
0.187 |
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Return to calculation section.