Hello dear all!
My first message for you in the new millenium !
In one of our 477¥s, I am having very low repetitive yield, especially for
peptides.
I checked the S2 delivery, ( it is now lower than the lowest level
recommended by the optimization rules ), I changed the TMA ( the RY improved
a little ) and also, I ran programs with different extraction conditions (
less and more post-TFA argon dry times ) but no differences were obtained,
eventhough these parameters are supposed not to have any influence in the
RY. What may gives any clue about, is that the glutamine, in
B-lactoglobulin, shows a some high deamidation effect ( about half of the
glutamine peak ).
This situation is the same, even with different reagents lotes.
Any suggestion would be welcome.
Thanks in advance for your help.
Happy 2000 !
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Susana Linskens
LANAIS-PRO
Junin 956
1113 Buenos Aires - ARGENTINA
linskens@qb.ffyb.uba.ar
FAX: 54-11-4508-3652
Phone: 54-11-4508-3651
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