If you can find it, or synthesize it, use a FITC-aldehyde and run the
reaction at a low pH (around 4-5) in the presence of CNBH3 or better yet
Pyridine-borane complex (20 mM, for each, overnite at 0-4 C. Otherwise, use
FITC-NHS or similar compound and try at a low pH as well. Chances are
though, you will end up modifying a number of internal Lys residues.
-----Original Message-----
From: Terry Stoming [mailto:TSTOMING@MAIL.MCG.EDU]
Sent: Tuesday, January 04, 2000 1:11 PM
To: Recipients of ABRF List
Subject: FITC
I need a quick clean protocol for labelling the N-terminus of a protein with
FITC
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