Dear colleagues,
we are doing 2D-PAGE on a Multiphor II/ Hoefer SE600 (using double gels)
system. Since we are currently setting up a proteome project for
Dictyostelium, I am now thinking about purchasing a high throuput 2D system
for these studies. I am especially interested in the second dimension and
have already thought about the Hoefer IsoDalt, BioRad's MultiProtean XL and
Genomic Solutions Investigator system. The system should be capable of
using 18 cm IPG strips and running 10 or 12 second dimension gels in
parallel.
What do you think about the three systems? Are there any more systems which
accomplish the mentioned conditions? What are their major pro's and con's?
The IsoDalt systems needs large amounts of running buffer, how does this
compare to the other two? As far as I could figure out, the cost are
approximately the same for all three systems so this should play a minor
role in the decision. Any information will be highly appreciated.
Yours sincerely,
Marcus Macht
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Dr. Marcus Macht
University of Cologne
Centre for molecular medicine - Service laboratory
Joseph-Stelzmann-Str. 52
50931 Cologne, Germany
Tel.: +49 221 478-6995
Fax: +49 221 478-6977
e-mail: Marcus.Macht@uni-koeln.de
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