Dear Marcus,
We have been using the Biorad Protean II for second dimension with 16 x 20
cm plates (18 cm IPG strips fit nicely including mol. wt. markers)....4
gels per tank and have two tanks connected in series to the recirculating
cooler unit. Approximately 2.5 litres of running buffer per tank. We can run
this twice a day to give us 16 gel throughput in a 24 hour period. The
multi-casting chamber permits 12 gels to be poured simultaneously. Uniform
heat transfer is an issue so we really crank up the flow on the cooler.
Actually pouring and running the gels is not the bottleneck the
staining/destaining is. Especially when using a silver staining protocol
there are so many steps, it really takes a lot of time.
----------------------------------------------------------------------
Gordon Alton, Ph.D.
Analytical/Protein Chemistry and Mass Spectrometry
Signal Pharmaceuticals, Inc.
5555 Oberlin Drive
San Diego, CA 92121
Email: galton@signalpharm.com <mailto:galton@signalpharm.com>
Phone: 858-558-7500 x8252
Fax: 858-623-0870
WWW: http://www.electriciti.com/signal <http://www.electriciti.com/signal>
----------------------------------------------------------------------
-----Original Message-----
From: Marcus Macht [SMTP:Marcus.Macht@uni-koeln.de]
Sent: Thursday, January 06, 2000 12:20 AM
To: Recipients of ABRF List
Subject: High troughput 2D
Dear colleagues,
we are doing 2D-PAGE on a Multiphor II/ Hoefer SE600 (using double
gels)
system. Since we are currently setting up a proteome project for
Dictyostelium, I am now thinking about purchasing a high throuput 2D
system
for these studies. I am especially interested in the second
dimension and
have already thought about the Hoefer IsoDalt, BioRad's MultiProtean
XL and
Genomic Solutions Investigator system. The system should be capable
of
using 18 cm IPG strips and running 10 or 12 second dimension gels in
parallel.
What do you think about the three systems? Are there any more
systems which
accomplish the mentioned conditions? What are their major pro's and
con's?
The IsoDalt systems needs large amounts of running buffer, how does
this
compare to the other two? As far as I could figure out, the cost are
approximately the same for all three systems so this should play a
minor
role in the decision. Any information will be highly appreciated.
Yours sincerely,
Marcus Macht
**************************************************************************
Dr. Marcus Macht
University of Cologne
Centre for molecular medicine - Service laboratory
Joseph-Stelzmann-Str. 52
50931 Cologne, Germany
Tel.: +49 221 478-6995
Fax: +49 221 478-6977
e-mail: Marcus.Macht@uni-koeln.de
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