We have been using a 20 gel DALT tank (originally from Hoefer, the Anderson
ISO-DALT System) along with the 20+ gel casting box for nearly ten years.
While this system uses large volumes of running buffer, it provides
excellent reproducibility and obviously high throughput. I am absolutely
happy with it. However, after all those years, our tank needs to be replaced
but the 20 gel system is no longer available. I've asked Amersham Pharmacia
Biotech reps about reviving the old larger system but there doesn't seem to
be much of a market. If there is indeed a broad interest in a 20 gel system
(anybody out there?), I guess we should let APBiotech know this in a
grass-roots kind of way. I hope they're subscribers!
Frank Witzmann
-----Original Message-----
From: Association of Biomolecular Resource Facilities
[mailto:abrf-request@aecom.yu.edu]On Behalf Of WSchick@aol.com
Sent: Thursday, January 06, 2000 7:01 PM
To: Recipients of ABRF List
Subject: Re: High troughput 2D with 20 Gel Hoefer?
The original Hoefer system had a ten gel tank. and a twenty gel caster.
Maybe
they would revive it for the new Proteomics interest. Cooling was
excellent,
and would be on their four gel tank also.
If I recall, they were able to supply a double gel configuration. Three
plates and two spacers, which would increase throughput.
Walt Schick
BioSepCo
> We can run
> this twice a day to give us 16 gel throughput in a 24 hour period. The
> multi-casting chamber permits 12 gels to be poured simultaneously.
Uniform
> heat transfer is an issue so we really crank up the flow on the cooler.
> Actually pouring and running the gels is not the bottleneck the
> staining/destaining is. Especially when using a silver staining protocol
> there are so many steps, it really takes a lot of time.
This archive was generated by hypermail 2b29 : Tue Jan 18 2000 - 11:43:39 EST