We are interested in synthesizing a peptide on an ABI 433A for use in a
fluorescent quenching assay, and we have a few specific questions:
1. Are there any solubility problems with using cartridges loaded with
FMOC-Abz-OH, FMOC-Tyr(m-NO2)-OH, FMOC-Lys(DABCYL)-OH, or FMOC-Asp(EDANS)
-OH?
2. Are there any problems associated with the unprotected hydroxyl on the
side chain of FMOC-Tyr(m-NO2)-OH?
3. Does the use of any of these compounds in peptides require that the
cleavage conditions be modified?
Thanks for any help.
Ray Paxton
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