Chris,
I have validated my microbore HPLC with a low molecular weight gel
filtration calibration kit (Pharmacia # 17-0442-01). There are 4 proteins,
each in a separate vial. I use a zorbax C3 column 1 mm x 15 cm, 3.5 um
particle diameter. Typically inject 1-5 ug and get quite nice 214 and 280
chromatograms with acetonitrile-water gradient (0.1% TFA in each).
----------------------------------------------------------------------
Gordon Alton, Ph.D.
Analytical/Protein Chemistry and Mass Spectrometry
Signal Pharmaceuticals, Inc.
5555 Oberlin Drive
San Diego, CA 92121
Email: galton@signalpharm.com <mailto:galton@signalpharm.com>
Phone: 858-558-7500 x8252
Fax: 858-623-0870
WWW: http://www.electriciti.com/signal <http://www.electriciti.com/signal>
----------------------------------------------------------------------
-----Original Message-----
From: chris halkides [SMTP:halkidesc@uncwil.edu]
Sent: Monday, January 10, 2000 12:09 PM
To: Recipients of ABRF List
Subject: laboratory exercise in hplc
Hello Everyone,
I am interested in developing a senior undergraduate lab
exercise
in the area of the hplc of proteins. Does anyone have any
suggestions for
a protein mixture to be be separated? literature references? I
would say
that the constraints are commercial availability of proteins and use
of
not-too-esoteric columns and reagents. I would think maybe a
mixture of
isozymes would be good (perhaps someone has a particular suggestion.
Thanks to all for any help.
Chris
Christopher Halkides
Dept. of Chemistry, UNCW
601 S. College Road
Wilmington, NC 28403-3297
(910) 962-7427
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