Chris: I have separated several isozymes and other proteins on HPLC. Actually a
good source for separation are root nodule extracts from soybeans. They have
abundant amount of heme-isoproteins. You can use a good ion-exchange column and
either straight Tris-buffer +/- salt or the BioCAD buffers for either anion or
cation exchange columns. In this regard, the BioCAD is a more powerful tool to
teach students about the joys and frustration of protein separations. At the
risk of sounding self promoting I am including the following reference from many
years ago: Sarath et al (1986) Analytical Biochem. 154: 224-231.
Another good set of proteins to separate are aminopeptidases in clarified
homogenates. These are easy to assay and one can treat the extract with
different inhibitors and see which isozymes get knocked out. There are many
other good examples in the literature. Good luck, Gautam Sarath
This archive was generated by hypermail 2b29 : Tue Jan 18 2000 - 11:43:39 EST