Dear Ray,
I would like to answer your questions concerning the amino acid derivatives
use for preparing FRET peptides:
1). Fmoc-Abz-OH, Fmoc-Tyr(m-NO2)-OH, Fmoc-Lys(DABCYL)-OH and
Fmoc-Asp(EDANS)-OH are soluble in DMF or NMP. There is a chance that the
delivery from the cartridge to the reaction vessel may not be complete due
to higher viscosity of the solution. Watch the delivery, if not complete,
allow more time for the delivery to the RV. Since these are expensive
derivatives, extra care is necessary.
2). The unprotected hydroxyl group is not very nucleophilic due to the
presence of the m-Nitro group. There should be no problem using
Fmoc-Tyr(m-NO2)-OH in the synthesis.
3). Use standard cleavage condition.
Anita Hong
AnaSpec, Inc.
2149 O'Toole Avenue, Suite F
San Jose, CA 95131
(408)452-5055 (phone)
(408)452-5059 (fax)
e-mail: service@anaspec.com
home-page: www.anaspec.com
............................................................................
........................
AnaSpec was recognized as a "Leader of the Pack"
in custom peptide synthesis by The Scientist.
............................................................................
........................
-----Original Message-----
From: PaxtonR@immunex.com <PaxtonR@immunex.com>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Monday, January 10, 2000 6:42 PM
Subject: PepSyn: fluorescently labeled peptides
>We are interested in synthesizing a peptide on an ABI 433A for use in a
>fluorescent quenching assay, and we have a few specific questions:
>
>1. Are there any solubility problems with using cartridges loaded with
>FMOC-Abz-OH, FMOC-Tyr(m-NO2)-OH, FMOC-Lys(DABCYL)-OH, or FMOC-Asp(EDANS)
>-OH?
>
>2. Are there any problems associated with the unprotected hydroxyl on the
>side chain of FMOC-Tyr(m-NO2)-OH?
>
>3. Does the use of any of these compounds in peptides require that the
>cleavage conditions be modified?
>
>Thanks for any help.
>Ray Paxton
>
>
This archive was generated by hypermail 2b29 : Tue Jan 18 2000 - 11:43:39 EST