Peter, Did you ever try increasing the back pressure after the
detector--like with a piece of smaller bore tubing--to see it that helped
alleviate the problem?
Deb McMillen
Institute of Molecular Biology
University of Oregon
EUgene OR
On Wed, 12 Jan 2000, Peter Hunziker wrote:
> Dear Pawel
>
> I had similar problems with other C4 colums. After the column has been
> used for some time a large peak always appeared at the same percentage
> of acetonitrile . It turned out that this peak was due to "air"-bubbles
> generated in the column or in the detector. I never could figure out
> what the reasons were (increased dead-volume in the column due to column
> compression, leaking fittings, etc..), the only thing that helped was to
> replace the column. It never happened with new or other types (C8, C18)
> of columns.
>
> Best regards
> Peter
>
> --
>
> ***********************************************
> Dr. Peter Hunziker
> Universitaet Zuerich
> Biochemisches Institut
> Winterthurerstr. 190
> CH-8057 Zuerich, Switzerland
>
> E-Mail: Peter.Hunziker@access.unizh.ch
> Phone: +41-1-635 55 20
> Fax: +41-1-635 68 05
> ***********************************************
> Visit our homepage: http://www.unizh.ch/~phunzi
> ***********************************************
>
>
This archive was generated by hypermail 2b29 : Tue Jan 18 2000 - 11:43:39 EST