Dear Mass speckers,
My lab has been beta testing the new software on the Voyager DE-STR and it looks like it's being sold now so I can talk about it. It has a lot of nice features that I don't want to talk about because I need a solution to a feature that they omitted.
Omission: You cannot overlay two spectrum files. And because of the way they wrote the software I don't think it is going to be something that they are going to be adding anytime soon. Unbelievable.
Example of why we want to overlay: We have a project we're working on that involves a bunch of GST fusion proteins. Ultimately we want to track, after an assay is run, which amino acids become phosphorylated.
No brainer, right.
Tryptic digest everything.
Shoot the GST no fusion.
Shoot the GST fusion.
Shoot the GST fusion putative phosphorylation.
Do a Protein Prospector MS-Fit on the GST fusion to make sure everything is as expected. Then the fun part: overlay the GST no fusion onto the GST fusion and isolate the unique peaks. These unique peaks now become your working template to compare to the phosphrylated sample. Overlay on the putative. Look for changes. End of story, go home and play with the kids.
But I can't overlay. Last PE answer: export chromatogram to Powerpoint. You have _got_ to be kidding me. Make sure that every parameter is exactly identical and then stick it in a graphics program?
So the question: What is the easiest way to do this? Are there other programs that the Data Explorer spectrum files can be exported to so one can overlay multiple files. Do we have to export them into Grams? I thought we had seen the last of that piece of crap but at least it could overlay.
Any simple suggestions?
thanks, Paul
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Paul Morrison JFB216 paul_morrison@dfci.harvard.edu
Molecular Biology Core Facilities
Dana-Farber Cancer Institute http://mbcf.dfci.harvard.edu
44 Binney Street 617-632-3082
Boston, MA 02115 fax 632-4814
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