Has anyone experienced Wako endo LYS-C cutting at amino acid residues other
than Lysine (K)? I have a client who is perplexed by my results and swears
there is a Lysine in the position I called a Leucine ( I stand by my
call). The endo LYS-C generated peptide sequence is as follows
(K) IGSQYDNYATC*L*AEHSLTEDDIFSIG...
Any experiences with this would be helpful.
Best Regards,
Suzanne Perry-Riehm
Suzanne C. Perry, Research Associate
NAPS Protein Sequencing and Peptide Mapping Laboratory
Biotechnology Department, University of British Columbia
CANADA
phone:(604)822-9688( direct) or (604)822-9662 (main) Fax:(604)822-0676
email:perry@interchange.ubc.ca
Visit our web page at : www.biotech.ubc.ca/naps/napshome.htm
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