Suzanne,
I have often observed cleavage after arginine as well as lysine
(lysine dominates, however). After prolonged incubation (over 8 h at
an enzyme-substrate ratio 1:100) arg-cleavage becomes significant.
However, I have not observed cleavage after other aminoacids.
Best reg's
Juhani
***************************************************
> Date: Thu, 13 Jan 2000 14:11:09 -0800 (PST)
> From: Suzanne Perry-Riehm <perry@interchange.ubc.ca>
> Subject: Peptide mapping and Endo LYS-C: odd cut sites
> To: Recipients of ABRF List <abrf@aecom.yu.edu>
> Has anyone experienced Wako endo LYS-C cutting at amino acid residues other
> than Lysine (K)? I have a client who is perplexed by my results and swears
> there is a Lysine in the position I called a Leucine ( I stand by my
> call). The endo LYS-C generated peptide sequence is as follows
> (K) IGSQYDNYATC*L*AEHSLTEDDIFSIG...
>
> Any experiences with this would be helpful.
>
> Best Regards,
> Suzanne Perry-Riehm
>
Juhani Saarinen
Protein Chemistry Laboratory
Institute of Biotechnology
University of Helsinki, Finland
e-mail jksaarin@operoni.helsinki.fi
Phone +358-9-708 59 414
Fax +358-9-708 59 416
Street adress:
Viikinkaari 9
FIN-00014, Helsingin yliopisto
PL 56
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