At 02:11 PM 1/13/2000 -0800, Suzanne Perry-Riehm wrote:
>Has anyone experienced Wako endo LYS-C cutting at amino acid residues other
>than Lysine (K)? I have a client who is perplexed by my results and swears
>there is a Lysine in the position I called a Leucine ( I stand by my
>call). The endo LYS-C generated peptide sequence is as follows
>(K) IGSQYDNYATC*L*AEHSLTEDDIFSIG...
Suzanne,
I can offer another explanation, which has come up at least twice around
here. File it under the "less obvious" causes of unusual Lys-C
behavior: Folks who aren't used to using the one-letter code for amino
acid residues are prone to certain common mistakes. One of these is using
or interpreting the letter L as lysine. MIght be a possibility here.
Rod Levine
NIH
Bldg 3, Room 106 MSC 0320
Bethesda, MD 20892-0320
email: rlevine@nih.gov
voice: 1 (301) 496-2310
fax: 1 (301) 496-0599
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