Dear Sandy: For what it is worth, I used to get a number of samples that were
formylated and I used to deblock using 0.6 to 1.0 M HCL in methanol at 40C for 4
to 24 hrs. There was usually some stagger, and I would suspect about 10 to 15%
deformylation, based on protein quantitation given to me. This treatment worked
well for most samples. However, background was a problem after 6 to 12
residues. I have not tested the outcome on the 494 or other higher sensitivity
sequencers. Without being presumptous, could some of these samples have a
non-formyl blocking group?? Gautam Sarath
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