Dear Leo,
Just yesterday, I attended the Benelux user meeting of the Micromass Q-TOF.
People presented there results they obtained with this instrument and it
was wonderful to see how diverse the use of this instrument was. There were
topics icluding quantification, exact mass easurement, non-covalent
interactions, LCMS, peptide, DNA, metabolites. Apart of some small details
in set up and software, everybody agreed it was a fine machine that has
several advantages over triple quad apart from neutrol loss capabilities
and related. It requires no special site conditions apart from some
thermostability. YOu just need a normal electricity supply and some outlets
for the gases and an internal cooling system. It fits in any medium size
lab room. We are very succesful in identifying proteins from 2D gels both
by LCMS as nanospray with it.
I must say that we obtained the instrument before there was the QSTAR
competitor (Sciex) and I did not test between the two. I think if you want
a very versatile instrument a QTOF is the right choise.
Bart
At 16:16 13/01/00 -0500, Leo Kei Iwai wrote:
>Dear Mass Spec users,
>
>
>
> We are thinking about buying a new Mass Spec equipment but I know
that there are dozens of models of equipments for sale now for different
uses, different sensitivity and different sizes.
> I'm planning to use it as a quality control of the peptides I
synthesize (i'm currently working with Micromass Maldi-tof and I'm doing
very well with it) but besides, I intend to use it to sequence proteins
from 2D gels and maybe to detect peptides eluted from MHC molecules.
> The question is, wich equipment would be better for these purposes
(Maldi, ES, Qtof...)? I know that Qtof is the newest model in the market
and it can detect peptides up to attomol range, but, can i use it as a
routine quality control equipment too?
> Also, if it is possible, I would like to have an idea of the other
different uses of the equipment you are using now and the size of it.
Actually we are going to move to a new building and I have to decide the
size of the room I will install the equipment. What will be the best size?
Besides a cool and dry room, is it necessary any kind of a special
electrical system for the equipment?
> Thank you in advance,
>
>Leo
>
>----------------------------------------------------------
>Leo Kei Iwai
>Chemist MSc
>MolBio PhD candidate
>Dept of Biophysics and Cell Biology
>Escola Paulista de Medicina - EPM - UNIFESP
>Lab of Transplant Immunology
>Instituto do Coracao - Incor - FMUSP
>----------------------------------------------------------
>
>
>
Dr. Bart Devreese
University of Gent
Laboratory of Protein Biochemistry and Protein Engineering
KL Ledeganckstraat 35
B-9000 Gent
BELGIUM
This archive was generated by hypermail 2b29 : Tue Jan 18 2000 - 11:43:39 EST