CHO analysis, sulfated GalNAc

From: James Pitt (jpitt@cryptic.rch.unimelb.edu.au)
Date: Thu Jan 20 2000 - 01:51:44 EST


A question for the glycobiologists:
If I have a glycoprotein containing sulfated GalNAc and I do TFA hydrolysis
followed by GC of the alditol acetates, does this show up in GalN(Ac) peak
ie is the sulfate hydrolysed? If so, is it quantitative? Thanks for any
thoughts.

James.
 

James Pitt,
Metabolic Screening & Biochemical Genetics Laboratory
Royal Children's Hospital,
Flemington Rd, Melbourne, Vic 3052, AUSTRALIA

Phone: 61 3 9345 5923
Fax: 61 3 9349 1819
Internet: http://www.rch.unimelb.edu.au/biochem/metlab.htm



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