Hi Patrick
I had the opportunity of testing both the IPGPhor and the BioRad IEF Cell.
My impressions can be summarized as follows
1) Both devices are nothing more than a programmable power supply plus a
peltier cooling element. Besides the fact that the prices for something
like that are ridiculously exaggerated, there is no way in which the two
devices can differentiate from each other a lot.
2) So the only differences are in the strips trays. Pharmacia has more
fancy ones, which supposedly conduct heat better (ceramic ?). BioRad has
more practical multi- well ones, that allows you to move around 12 strips
at the time. Considering that the Pharmacia trays are also fragile and
expensive, this seems to me to give BioRad a slight advantage, so I bought
the BioRad device
3) You should however consider that the size of the tray is adapted to the
length of the strips each company produces, which especially for long
strips is different (17 cm BioRad, 18 cm Pharmacia). You may want to check
out which strips you prefer, basing on the different pH ranges offered by
the two companies.
Otherwise there is little difference, and you should stick to the company
which you trust the most. Consider also that both devices are in principle
not superior to the old Multiphor system, just faster and easier to
operate.
Good Luck
P.S. For Bart Devreese ; you should definitely use oil while rehydrating ,
too !!!
>Hi everybody,
>
>I apologise beforehand as I know this has been asked ad nauseum, but we need
>to make a decision within the next week on whether to purchase the IPGPhor
>or the Bio-Rad Protean IEF cell for 2D 1st dimension. I've searched the
>archive and found enough about the IPGPhor, but would be very interested to
>hear from anybody who has had experience with Bio-Rad's IEF cell. Also, the
>advantages/disadvantages between the two units would be a great help!
>
>Many thanks!
>
>Patrick Tam
>Oncology Research Centre
>Level 2 Clinical Sciences Building
>Prince of Wales Hospital
>Barker St
>Randwick NSW 2031
>AUSTRALIA
>Ph: 02 9382 2617
>Fax: 02 9382 2629
>email: p.tam@student.unsw.edu.au
>______________________________________________________
>Get Your Private, Free Email at http://www.hotmail.com
Manfredo Quadroni
Protein Chemistry Laboratory
ETH Zentrum
Universit”tstrasse 16
8092 Z¸RICH
SWITZERLAND
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