Hi Colleagues,
I have benefitted a lot form the discussions that go on in this group , and
would like to avail of your collective wisdom.
I am faced with a peculiar problem with the synthesis of two peptides that
are designed to identify a immunological epitope. The sequences are as follows,
TTVYPPSSTAK and TVYPPSSTAK.
I have synthesized these two sequences twice on the Pioneer with two different
batches of the acid Lys resin. My HPLC shows two peaks almost on the top of each
other. We tried purifying the major peak on a prep column, but analysis of the
"pure" peaks seem to indicate that nothing has changed. They look identical to
the crude HPLC. The mystery is that the MALDI of both the samples do not
indicate anything untoward. The only peaks are that of the peptide.
Is it possible that one of the amino acids might be a racemic mixture? All
my reagents are form PE Biosystems.
Will AAA tell me anything if there were isomers.
I have calibrated both my analytical and prep c18 columns.
Thanks.
Radhika.
City of Hope, Duarte CA.
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