Dear Joseph,
If you want to quantify the amount of peptide in your peptide sample
you may perform amino acid composition analysis. Normally the peptide
content ranges between 60-90% depending f.ex. on the vacuum on your
lyophilizer (if you dry your peptide in several batches, they likely
differ in peptide content).
To quantify the residual 10-40% mostly consisted of water and
counter-ions, gas chromatography may give an answer.
Henriette
>What is the best way to quantitate the amount of H2O and counter-ion in
>a sample of lyophilized peptide?
>
>Are esters stable to HF cleavage?
>
>--
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>Joseph A. Fuselier
>Peptide Research, SL12
>Tulane University School of Medicine
>1430 Tulane Avenue
>New Orleans, Louisiana 70112 USA
>504.588.2296
>(fx) 504.584.3586
>devildog@mailhost.tcs.tulane.edu
>
>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Henriette A. Remmer, Ph.D
Director
Protein Sciences Facility
Biotechnology Center
University of Illinois
311/315 Noyes Laboratory, Box 62-1
505 S. Mathews Ave.
Urbana,IL 61801
Tel: (217) 333-4695 (Lab) (217) 333-3841 (office)
Fax: (217) 244-1142
homepage: http://www.life.uiuc.edu/biotech/protein_sc.html
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