Radhika,
No.
At room temp there will always be an equilibrium mixture of the two
isomers. As soon as you separate one from the other, the system eqilibrates
and the mixture reforms. Unless something in your biological system
stabilizes one over the other, the mixture will be present. The biological
system may select for one over the other, activity-wise, but that may be
hard to tell. To add insult to injury, if your biological system consumes
one preferentially, it will continue to reform from the other because of
the equilibrium. That may make for a pretty sticky (as in difficult to
interpret) assay.
Greg
>Thanks to everybody who resolved this problem for me.
>I appreciate all the input.
>I do have one reulting question however, these peptides will be used in a
>biological assay done at room temperature. Will this affect the formation
>of one
>isomer versus the other. I would assume that the trans is more stable than the
>cis at higher temperatures, and vice versa. Is it at all possible to separate
>these kind of isomers?
>Thanks a lot.
>Radhika.
>City of Hope, Duarte.
Gregory A. Grant
ggrant@molecool.wustl.edu
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