Dear Dr. Lombardo,
thanks for your reply.
your problem is obviously caused by the z-height. are you sure that the
liquid is spraying during liquid delivery? or is the liquid coming out
during the nitrogen blowing? there are two possibilities.
The former: during the liquid delivery steps the needle is to close to
the tube lid and the buffers are hanging on the lid.
The latter: during the nitrogen delivery the sealing is not perfect and
of course this is in contrats a gas bubbling.
To fix the problem: please refer to chapter "adjusting the inject
position of the needle", at page 10.
[add comment: in the exchange kit manual]
we stated that the z-home value 410 will be okay, but maybe not in all
cases (in fact you are the first one who is giving us this error
message).
please go through the described procedure and try another higher value
(top is 0 bottom around 1250 for the z-axis), so let's say 415.
Iam just writing a special note for adjusting the right z-position. as
soon as it is ready I will send it to you by email.
please keep me informed
Kind regards
Joern Kirchhuebel
::::::::::::::::::::::::::::::::
Dr. Joern Kirchhuebel
Product Specialist Life Science
ABIMED Analysentechnik GmbH
Raiffeisenstraŝe 3
40764 Langenfeld
Germany
................................
phone +49 2173 8905-38
fax +49 2173 8905-77
::::::::::::::::::::::::::::::::
----Original Message-----
From: Christian R. Lombardo [SMTP:crl@burnham-inst.org]
Sent: Tuesday, January 25, 2000 5:36 PM
To: kirchhuebel@abimed.de
Subject: DigestPro
Dear Jorn,
We received the replacement lid included with the exchange kit
for the
DigestPro that you sent. Thank you. I reprogrammed the
controller and
installed the new lid as instructed in the manual. We are still
having a
very serious problem. During the addition of the reagents, the
liquid
comes out of the tops of the reaction tubes. Not all of the
liquid goes in
the tubes and much of it stays at the top. On our first attempt
at
performing digests with the DigestPro, there was liquid spilling
over the
top. The problem is that the acetonitrile used in the digest
reaction
destroys the plastic polycarbonate lid. This is what happen
last time.
This has already begun to happen with the new lid!! I would
appreciate any
suggestions you may have regarding how to prevent this problem.
Thanks in advance.
-Chris-
***********************************
Christian R. Lombardo, PhD
Director, Molecular Analysis Facility
The Burnham Institute
10901 North Torrey Pines Road
La Jolla, CA 92037
858-646-3108
crl@burnham-inst.org
***********************************
Joern Kirchhuebel
::::::::::::::::::::::::::::::::
Dr. Joern Kirchhuebel
Product Specialist Life Science
ABIMED Analysentechnik GmbH
Raiffeisenstraŝe 3
40764 Langenfeld
Germany
................................
phone +49 2173 8905-38
fax +49 2173 8905-77
::::::::::::::::::::::::::::::::
>-----Original Message-----
>From: Christian R. Lombardo [mailto:crl@burnham-inst.org]
>Sent: 25 January 2000 17:14
>To: Recipients of ABRF List
>Subject: DigestPro
>
>
>Good Day,
>
>We are having a problem with our DigestPro instrument. For those of
you
>out there in ABRFland that have this instrument, I'd appreciate your
>feedback.
>
>The problem is this. During the addition of the reagents by the robot,
the
>liquid spills out of the tops of the reaction tubes. Not all of the
liquid
>goes in the tubes and much of it stays at the top. This happens
regardless
>of the gel percentage. We also cut the gel band into 4 to 6 1mm3
chunks
>(no crushing). So, I do not think that plugging of the hole in the
bottom
>is the issue. What happens is that the acetonitrile used in the digest
>reaction destroys the plastic polycarbonate lid when the liquid spills
over
>the tops of the tubes. We replaced the polycarbonate lid included
with
>the exchange kit for the DigestPro so that the tubes without adaptors
can
>be used. This problem has already begun to happen with the new lid!!
Of
>much greater concern is the inconsistency of liquid delivery during the
>washing and reduction/alkylation steps. This probably relates to why
some
>digests work and others, mysteriously, do not. Any advice would be
greatly
>appreciated.
>
>Thanks in advance.
>
>-Chris-
>
>***********************************
>Christian R. Lombardo, PhD
>Director, Molecular Analysis Facility
>The Burnham Institute
>10901 North Torrey Pines Road
>La Jolla, CA 92037
>858-646-3108
>crl@burnham-inst.org
>***********************************
>
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