Dan,
We have the Fluor-S MultiImager System from Bio-Rad, along with PDQuest and
Melanie for 2D imaging, analyzing, and databasing 2D gels. I would
recommend this imaging system.
A few points Fluor-S has:
1.) CCD camera resolution of 1,340 X 1,040 pixels. This can provide double
the resolution power over more typical CCD-based imaging systems.
2.) The dynamic range of 3 orders of magnitude for increased quantitation.
Instead of a standard 8-bit grayscale, with 256 levels of gray, the Fluor-S
has a dynamic range of 12-bits, or 4,096 levels of gray.
3.) The unique scanning and epi-illumination system for both UV and white
light can provide high-sensitivity imagining of all: fluorescent, chemi-, or
colorimetric 2D gels.
4.)Interchangeable zoom lenses for large and small scale gels, and many
filters for many fluorescent dyes.
The software for both capture and analysis are very powerful tools and are
very user-friendly.
Tech service seems to be OK if you contact the right people at Bio-Rad. The
800 number is useless, but the imaging specialist I deal with is very
helpful.
Jason Marchese
AstraZeneca R & D Boston
One Innovation Drive
Worcester, MA 01605
jason.marchese@astrazeneca.com
-----Original Message-----
From: Dan.Kirby@praecis.com [mailto:Dan.Kirby@praecis.com]
Sent: Monday, January 31, 2000 11:03 AM
To: Recipients of ABRF List
Subject: 2D Gel Scanning
We are considering the purchase of equipment for qualitative and
quantitative
scanning of 2D gels. I would appreciate any suggestions and/or comments from
users. Thanks in advance.
Dan Kirby
Praecis Pharmaceuticals
1 Hampshire Street
Cambridge, MA 02139
dan.kirby@praecis.com
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