Hi Andrew,
We do NaAc/EtOH ppt. with both 96-well and 384-well plates and resuspend in
water on a normal basis for reactions used on the 3700.
- George
At 05:19 PM 2/1/00 +0100, Andrew.Walding@astrazeneca.com wrote:
>To all 3700 users
>
>Do you have any preferences to protocols when precipitating your sequencing
>reactions for use on the 3700? (e.g. no salt?)
>Also, have you had much success with resuspension in water (as opposed to
>formamide)?
>
>Thanks in advance
>
>Andrew
>
>_______________________________________________________________
>AstraZeneca R&D Charnwood
>Molecular Biology, Bakewell Road, Loughborough, Leics, LE11 5RH, England
>Tel: +44 (0)1509 644213 Fax: +44 (0)1509 645557 Mobile: +44 (0)787
>9615913
>andrew.walding@astrazeneca.com
>
________________________________________________________________
George Grills
Director
DNA Sequencing, Genotyping, Oligonucleotide, and GeneChip Microarray
Facilities
Albert Einstein College of Medicine
713 Ullmann Building
1300 Morris Park Avenue
Bronx, New York 10461-1602
Tel: (718) 430-2657
Fax: (718) 430-8778
E-mail: grills@aecom.yu.edu
DNA Sequencing: http://leper1.ca.aecom.yu.edu/dnacore
Oligonucleotide: http://sequence.aecom.yu.edu/oligo
________________________________________________________________
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