Hi Tim,
In our hands, water gives longer lengths of read than formamide.
- George
At 09:09 AM 2/2/00 -0500, Timothy D. Howard wrote:
>We do a similar precipitation, but resuspend in Hi-di formamide. Does water
>work just as well?
>
>Tim
>
>Tim Howard, Ph.D.
>Center for the Genetics of Asthma & Complex Diseases
>University of Maryland, Baltimore
>108 N. Greene St.
>Baltimore, MD 21201
>Ph: (410) 706-1690
>
>
>Hi Andrew,
>
>We do NaAc/EtOH ppt. with both 96-well and 384-well plates and resuspend in
>water on a normal basis for reactions used on the 3700.
>
>- George
>
>
>
>At 05:19 PM 2/1/00 +0100, Andrew.Walding@astrazeneca.com wrote:
>>To all 3700 users
>>
>>Do you have any preferences to protocols when precipitating your sequencing
>>reactions for use on the 3700? (e.g. no salt?)
>>Also, have you had much success with resuspension in water (as opposed to
>>formamide)?
>>
>>Thanks in advance
>>
>>Andrew
>>
>>_______________________________________________________________
>>AstraZeneca R&D Charnwood
>>Molecular Biology, Bakewell Road, Loughborough, Leics, LE11 5RH, England
>>Tel: +44 (0)1509 644213 Fax: +44 (0)1509 645557 Mobile: +44 (0)787
>>9615913
>>andrew.walding@astrazeneca.com
>>
>
>________________________________________________________________
>
> George Grills
> Director
> DNA Sequencing, Genotyping, Oligonucleotide, and GeneChip Microarray
>Facilities
> Albert Einstein College of Medicine
> 713 Ullmann Building
> 1300 Morris Park Avenue
> Bronx, New York 10461-1602
>
> Tel: (718) 430-2657
> Fax: (718) 430-8778
> E-mail: grills@aecom.yu.edu
> DNA Sequencing: http://leper1.ca.aecom.yu.edu/dnacore
> Oligonucleotide: http://sequence.aecom.yu.edu/oligo
>________________________________________________________________
>
>
>
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