HPLC - passivation

From: Richard F. Cook (cook@MIT.EDU)
Date: Thu Feb 03 2000 - 16:59:53 EST

Dear ABRFers,

Does any one have a good HPLC passivation SOP?

I'd like someting that I can run through the whole system from buffer
bottle to flow cell with out doing damage. I'd like to do this to a HP1100.
I've seen some talk on the ABRF List using 6N HNO3 for 30' at high flow
rate but that seems too severe. HP tech support suggests 1N HNO3 but they
do not have a time period for that step.

Thanks in advance.

Dick

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