Hi, all,
Has anyone seen cleavage between His and Pro (sequence xxHPxx) with Endo
Lys C cleavage or under acidic conditions (I'm thinking specifically
about the HPLC fractions sitting around in 0.1% TFA/Accn until we ran
them on the protein sequencer)?
If this peptide bond (HP) is sturdy, then I imagine that we have a
Lysine in our protein sequence rather than the predicted His--but then I
thought that KP could not be cut by endo Lys C? Do I have this right?
Thanks for any input,
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97403
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Take care
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