Dear colleagues,
On the paper published in ABRFNews, March 98 by Walsh et al, "Setting Up Two
Dimensional Gel Electrophoresis for Proteome Projects", there is an anode
electrode buffer ( only 0.75 M Tris pH 8.8 and 0.005% sodium azide: no
glycine, no SDS) and a cathode electrode buffer (192 M Glycine, 0.1% SDS, pH
8.3 adjusted with Tris). The anode buffer can be re-used during 20 gel runs.
Considering the large volumes of buffer used in the second dimension anode
tanks, this proposal makes a great economy. I would greatly appreciate your
experience using this running buffer (any influence on the quality of the
separation) that I found highly attractive.
Thanks in advance for your time and advice,
Lila Castellanos-Serra
Division of Physical Chemistry
CIGB Havana
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