Hi ABRF ers,
Hope somebody can help me with these questions.
a) Is there a convenient reference with all the amino acid UV absorbances?
b) what is the the generally accepted quantity of sample(peptide) that can be
injected on a analytical column(4.6x250mm) . Over what absorbance level( in mAu)
is it considered to be "over loading "the column.
I hope these questions don't sound stupid!!!!
Thanks.
Radhika
City of Hope, Duarte.
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