> with 150mM salt and the fun began.
Janelle Lauer-Fields
That is the culprit I think. HPLC don't like salt (I had a method using
1.5M KCl) it just eat up slowly the pump seals and block the frits. The
buffer leak slowly at the back of the pump seal through the piston, the
solvent evaporate leaving rasor blade salt crystal. It normally leave a
black residues on the frits of the output pump valve. A way to avoid that is
to wash the back of the seal with a lot of water, daily. I don't know about
the HP pumps but with the 125/126 from Beckman they have a port to circulate
water at the back seal location. I used to use a peristaltic pump to have a
continuous circulation of water at the back of the piston seal.
Hope it help
Jean Lagueux
Proteotech
4690 Clara-Brousseau
Cap-Rouge,G1Y 3N1, Qc, Canada
tel: (418) 561-6480
fax (418) 652-1665
This archive was generated by hypermail 2b29 : Wed Feb 09 2000 - 10:35:56 EST