Dear Radhika,
no suggestions regarding the palmitoylation issue, however, as to the
second point there is a paper published by Fernando Albericio and
Ernest Giralt that may be of help: "Convergent solid-phase peptide
synthesis. 12. Chromatographic techniques for the purification of
protected peptide segments", Int J Pept Protein Res 1995
Aug;46(2):119-33. They describe modified reversed-phase
chromatographic techniques in which DMF was added to the water and
acetonitrile mixtures used as eluents.
Luisa
----------------------------------------------------------------------
Luisa Rusconi
Pharmacia & Upjohn
Dept. of Biology
Laboratory of Biochemistry
Viale Pasteur, 10
20014 Nerviano (MI)
Italy
______________________________ Reply Separator _________________________________
Subject: Pepsyn:Palmitic acid
Author: "Radhika Krishnan"<rkrishnan@smtplink.Coh.ORG> at Internet-europe
Date: 04/02/2000 9:57 AM
Hi Everyone,
Just wondering if there are any lipopetide generators out there. I have been
having problems dissolving Palmitic acid in 1:1, 1M DIEA and 0.5M HBTU/HOBt (
Pioneer activators). I get a brown coagulated mass, that dissolves after some
warming up in a water bath. When I add the solution to the resin however it
coagulates up too, but does liquefy on warming up. I am using the normal mole
excess that is recommended and doing the coupling manually.
How would you generate symmetric anhydride of the Palmitic acid and what
activators would be used for that coupling.
On a related note. has anybody used DMF for analytical HPLC seperations and
what wavelength would you monitor the run. We do have a PDA.
Hope I can get some hits on these questions.
Thanks in advance to any body with suggestions.
Radhika.
City of Hope, Duarte.
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