Dear Jeni
Is your system fitted with the thermostated column compartment?
In my 1100 all the columns are now fitted with in-line frits,
because I find that that even in 'normal' RP-HPLC use they tend
to block fairly quickly, necessitating a change about once a
week. Examination of the blocked frits often shows that they
have collected small pieces of 'rust'? I suspect that they may
be coming from the thermostated compartment - they look like
sprayed cast iron blocks to me. Running salt solutions through
these blocks is therefore bound to accelerate your problems - if
what I suspect is right that is - anyone from Agilent care to
comment?
Good Luck, JG.
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Dr J. Gray
University of Newcastle Upon Tyne
Molecular Biology Unit
3rd Floor Cookson Building
Framlington Place
Newcastle NE2 4HH
United Kingdom.
Tel. / FAX : +44 (0) 191 222 8612
Email : joe.gray@newcastle.ac.uk
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