Dear ABRF members:
I need some advice from you:
I ran some plasmid samples together with the PGEM control from PE, on a ABI
377.
I use bigdyes;after cycle-sequencing samples were frozen and purified next
day on centrisep columns. I evaporate on vaccuum centrifuge.
I applied 1.5 ul of re-suspended samples, in a 24 comb.
I use a 19.1 policarylamide solution
Unfortunately, although I can see the signal of some sequence on the gel,
the software makes no analysis "weak signal, analysis failed".
What could possible be wrong??
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