I wonder if anyone can help me with this - we don't have much experience of
working with lipid, but we currently have quite large amounts of lipid
present in recombinant protein samples we are trying to develop rpHPLC
separations for. How will the lipid affect reversed phase? should we
delipidate first? (there seems to be a problem with our protein
precipitating when we try chloroform/methanol washing). Will phospholipid
(we assume that's what it is) co-elute with protein in gradients of
acetonitirile on C8/C18 columns? Can anyone recommend any references?
Thanks in advance for any advice,
yours in ignorance of all things lipid,
Derek Ellison
Medeva Development
Speke
Liverpool
L24 9GR
Phone: 44 151 705 5442
Fax 44 151 705 5189
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