RE: Why won't a PCR primer work for sequencing

From: wagner yotov (yotovw@ERE.UMontreal.CA)
Date: Tue Feb 08 2000 - 10:44:40 EST

Next message: Mark W. Crankshaw: "RE: phosphorothioate oligos"
Previous message: Frank Bruce: "RE: DNASyn:Sulfur backbone oligos"
In reply to: Ken Idler: "Re: Why won't a PCR primer work for sequencing"
Next in thread: Robert Lyons: "Re: Why won't a PCR primer work for sequencing"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

Mark,

In our hands the non-desalted oligos (e.g. those ordered "standard purity"
from GIBCO-BRL) worked in PCR but often did not work for sequencing. After
desalting (by ethanol precipitation) they worked OK.

Regards,
wagner

Next message: Mark W. Crankshaw: "RE: phosphorothioate oligos"
Previous message: Frank Bruce: "RE: DNASyn:Sulfur backbone oligos"
In reply to: Ken Idler: "Re: Why won't a PCR primer work for sequencing"
Next in thread: Robert Lyons: "Re: Why won't a PCR primer work for sequencing"
Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]

This archive was generated by hypermail 2b29 : Wed Feb 09 2000 - 10:35:56 EST