John,
I have not tried it but there is a paper by Tam which uses this approach -
Lu, Y et al, Molecular Immunology,1991, 28(6), 623-630. In summary,
an N-chloroacetylated octavalent Lys core and a synthetic peptide
with an N-terminal Cys are coupled through the Cys to give a MAP with
the carboxyl terminus exposed.
>I recently had a request to produce a MAPs peptide with an
>octavalent Lysine core. However this person needs the C-terminus
>of the peptide exposed
>and the N-terminus of the peptide covalently linked to the
>poly-lysine core.
>
>Do any of you have experience with this type of a request or some
>good suggestions as to an approach to produce such a peptide?
>
>Thank you for your attention.
>
>John Phipps
>
>
>
>
>
>
>
>John Phipps
>Manager
>Macromolecular Resources
>Colorado State University
>jphipps@mmr.bmb.colostate.edu
>ph (800)-491-0424
>fax (800)-436-2329
Bernie McInerney
Australian Proteome Analysis Facility
Level 4 Building F7B
Macquarie University NSW 2109 Australia
Tel : 61 2 9850 6207
Fax : 61 2 9850 6200
Email : bmcinern@proteome.org.au
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