Dear John,
most probably, the MAP peptide will be used for antibody production and the
peptide is the C-terminal part of the protein. In such cases, I certainly
advise to use simple peptides and to couple them, either through the
N-terminal amino group (in case there is no lysine present in the sequence)
or by adding a cysteine N-terminally and coupling through its SH group, to
a protein carrier like KLH or BSA. This is in my opinion the best way to
proceed.
If really a MAP peptide is required, I think the only way is to synthesize
the free peptide and couple it after cleavage and purification through the
N-terminal amino group, or again through an added cysteine's SH group to
the MAP core. But there is no advantage in doing this.
I hope this helps.
Gottfried Proess
Tel.: + 32 4 366 01 50 Fax : + 32 4 365 16 04
E-Mail: g.proess@eurogentec.com
Web-Site : http://www.eurogentec.com <==== visit it !
-----Message d'origine-----
De: John Phipps [SMTP:jphipps@mmr.bmb.colostate.edu]
Date: mercredi 9 fÈvrier 2000 00:09
¿: Recipients of ABRF List
Objet: peptide synthesis
I recently had a request to produce a MAPs peptide with an octavalent
Lysine
core. However this person needs the C-terminus of the peptide exposed
and the N-terminus of the peptide covalently linked to the
poly-lysine core.
Do any of you have experience with this type of a request or some good
suggestions as to an approach to produce such a peptide?
Thank you for your attention.
John Phipps
John Phipps
Manager
Macromolecular Resources
Colorado State University
jphipps@mmr.bmb.colostate.edu
ph (800)-491-0424
fax (800)-436-2329<< Fichier: ATT00001.htm>>
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