Ken,
Went to the ooold files and pulled out a July 1979 protocol. The typewriter
ink had held up, but I have to admit that the pages look like they are
yellowing a bit. We used to take 1 mm gel slices (cut from tube gels with an
electric Hoefer gel slicer), added 1 ml of 15% hydrogen pyroxide to each gel
slice and incubated overnight at 70 deg C. Cooled the samples and added 2 mls
of Aquasol-2 to each sample, shook them vigorously--the main thing here will be
to assure that the scintillation cocktail that you use will mix well with the 1
ml of 15% hydrogen pyroxide--we were using the larger glass scintillation vials
in those days.
Deb McMillen
Institute of Molecualr Biology
University of Oregon
Eugene OR 97403
Kenneth Williams wrote:
> One of our users would like to dissolve several CB stained SDS gel bands so
> they may be subjected to scintillation counting to quantify tritium labeled
> proteins. If anyone has a reference or favorite protocol for dissolving and
> counting SDS gel bands please post it or send it to me at
> kenneth.williams@yale.edu
>
> Thanks very much,
> Ken Williams.
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