Dear Radhika,
As I remember, fatty acids are well soluble in methylene chloride, but
do not chill the solution during activation. I had the experience of
obtaining succinimide ester of stearic acid with DCC, think any
coupling reagent should work. From the colleagues' experience, some
palmitoylated peptides elute from RP columns and some don't. I'm not
sure adding DMF would help, perhaps, trying less hydrophobic phases
(C1, C2...) or using direct phase would help (I purified my
lipopeptides on conventional glass columns with silica gel using
RP-HPLC only for analysis).
Hope this helps
Vladimir
On 04/02/2000, Radhika Krishnan wrote:
> Hi Everyone,
> Just wondering if there are any lipopetide generators out there. I have been
> having problems dissolving Palmitic acid in 1:1, 1M DIEA and 0.5M HBTU/HOBt (
> Pioneer activators). I get a brown coagulated mass, that dissolves after some
> warming up in a water bath. When I add the solution to the resin however it
> coagulates up too, but does liquefy on warming up. I am using the normal mole
> excess that is recommended and doing the coupling manually.
> How would you generate symmetric anhydride of the Palmitic acid and what
> activators would be used for that coupling.
> On a related note. has anybody used DMF for analytical HPLC seperations and
> what wavelength would you monitor the run. We do have a PDA.
> Hope I can get some hits on these questions.
> Thanks in advance to any body with suggestions.
> Radhika.
> City of Hope, Duarte.
Vladimir Titov
Bokiron Ltd., Moscow, Russia
This archive was generated by hypermail 2b29 : Mon Feb 14 2000 - 16:05:59 EST