We just found out about a great list of peptide, protein, and CHO
standards. The tables list the source, mass, and company where the
standards can be purchased. These lists were prepared by Thermo
BioAnalysis Ltd. and brought to our attention by LeeAnn Higgins.
See:
http://www.maldi.com
and request Application Note 9 "Recommended Mass Calibrants for Matrix
Assisted Laser Desorption Mass Spectrometry"
-Lowell H. Ericsson, Dept. of Biochemistry, U. of Washington, Seattle, WA
P.S. For many years we have used apomyoglobin from horse skeletal muscle
from Sigma as our principle checking standard which we dissolve in 5% HAc,
50% MeOH. Cat# A-8673. Avg. MW MH+ 16,951.5
On Tue, 8 Feb 2000, Bill Enslow wrote:
> To the mass speccers,
> We just got a new MALD/I and a new LC ion-trap. I am going to
> purchase the standards apomyoglobin, cytochrome c, melittin, BSA and
> angiotensin 1. I was going to purchase them from Sigma. Has anyone has a
> bad experience (degredation, contamination, etc,) with Sigma proteins as
> standards? Is there a better place to get them?
> Thanks for your wisdom,
> Bill
>
> ------------------------------------------------------------------------------
> Bill Enslow
> Protein Sequencing Technician
> Cornell BioResource Center
> Rm 143 Biotechnology Building
> phone: 607-254-4848
> fax: 607-254-4847
> ----------------------------
>
>
>
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