Recipients of ABRF List <abrf@aecom.yu.edu>
MIME-Version: 1.0
Content-Type: text/plain; charset=us-ascii
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Sender: Association of Biomolecular Resource Facilities <abrf-request@aecom.yu.edu>
Precedence: bulk
Errors-To: abrf-request@aecom.yu.edu
Jeni, et al.
I am not so sure about the 0.1% methanol. Some years back I had definitely
something growing in the wash solvent lines and my wash solvent was 20%
methanol! Keeping light out (amber bottles or wrapped in aluminum foil) seemed
to help. Perhaps the biologists who grow bugs (and algae?) for a living might
be able to shed some light (no pun intended) on this.
Ioannis Papayannopoulos
AstraZeneca R&D Boston
(Still in) Worcester, MA
--- Jeni Lauer-Fields <jlauer@ACC.FAU.EDU> wrote:
> Hi,
>
> Sorry to bother everybody again, but I have some more information. I received
> an
> email from a fellow ABRFer and a call from an Agilent scientist saying that
> the
> addition of 0.1% methanol to all aqueous buffers should solve the problem of
> bacterial growth without affecting chromatography.
>
> I was going to add sodium azide, but methanol is much easier to deal with.
> It's
> a tip I felt we could all benefit from.
>
> Thank you again,
>
> Jeni
>
> Janelle Lauer-Fields
> Department of Chemistry & Biochemistry
> Florida Atlantic University
> 777 Glades Road
> Boca Raton, Florida 33431
> 561-297-2094/Fax 561-297-2759
>
>
__________________________________________________
Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.
http://im.yahoo.com
This archive was generated by hypermail 2b29 : Thu Mar 16 2000 - 10:06:51 EST