Dear Jeni,
Sodium azide has a reasonable absorbance in UV wich may affect your
measurements. I am not sure, however, that 0.1% methanol may suspend
bacterial growth: you know, bacteria work in up to 12% ethanol (dry
wine concentration), and I know that home-made wines may contain
reasonable amounts of methanol (though I don't know exact percentage).
However, 0.1% TFA does not tend to "flourish" (in contrast to
phosphate buffers). Also, it is good to fill the HPLC system and the
column with a solvent like 80% methanol or acetonitrile when not in
use.
Vladimir
On 16/02/2000, Jeni Lauer-Fields wrote:
> Hi,
> Sorry to bother everybody again, but I have some more information. I received an
> email from a fellow ABRFer and a call from an Agilent scientist saying that the
> addition of 0.1% methanol to all aqueous buffers should solve the problem of
> bacterial growth without affecting chromatography.
> I was going to add sodium azide, but methanol is much easier to deal with. It's
> a tip I felt we could all benefit from.
> Thank you again,
> Jeni
> Janelle Lauer-Fields
> Department of Chemistry & Biochemistry
> Florida Atlantic University
> 777 Glades Road
> Boca Raton, Florida 33431
> 561-297-2094/Fax 561-297-2759
Vladimir Titov
Bokiron Ltd., Moscow, Russia
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